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偏性C 1化合物資化性菌Methylomonas sp. Jが生産するglutamine synthetaseの精製と性質
https://swu.repo.nii.ac.jp/records/2367
https://swu.repo.nii.ac.jp/records/2367755555ab-4572-4e4a-a928-09fe6882c1b6
名前 / ファイル | ライセンス | アクション |
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KJ00004311150.pdf (865.3 kB)
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Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-02-16 | |||||
タイトル | ||||||
タイトル | 偏性C 1化合物資化性菌Methylomonas sp. Jが生産するglutamine synthetaseの精製と性質 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | PURIFICATION AND SOME PROPERTIES OF GLUTAMINE SYNTHETASE FROM METHYLOMONAS SP. J | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10474552 | |||||
著者 |
松本, 孝
× 松本, 孝× Matsumoto, Takashi |
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抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | An obligate methylotrophic bacterium, Methylomonas sp. J is able to grow on only two C-1 compounds, methanol and methylamine. In our previous study (2), it was suggested that the glutamine synthetase was a preferred enzyme as the nitrogen assimilation in the cells, when nitrate and methanol were the respective nitrogen and carbon sources for this micro-organism. While, when methylamine was only nitrogen and carbon source, it was shown that two enzyme activities, glutamine synthetase and NADP-depenndent glutamate dehydrogenase activities were detected. From these observations, much interest has focused on the regulation of the glutamine synthetase activity in assimilation of nitrogen. The aim of this study was to characterize glutamine synthetase from Methylomonas sp. J in different nitrogen sources. so, at first, glutamine synthetase of this micro-organism was purified to homogeneity from the cells grown in synthetic medium of methanol and nitrate as carbon and nitrogen sources, respectively. The purified enzyme had a molecular weight of 580,000 and a subunit size of 55,000. The studies of the effect on the catalytic properties by snake venom phosphodiesterase treatment suggest this enzyme is regulated by adenylation/deadenylation. The release of AMP from the enzyme was also detected by the treatment. Some other properties of the enzyme, such as Km for each substrate, inhibition by some metabolites and effect of metal-ions were determined and compared to those in the case of other bacterial enzymes. | |||||
書誌情報 |
昭和女子大学大学院生活機構研究科紀要 en : Bulletin of the Graduate School of Human Life Sciences, Showa Women's University 巻 8, p. 55-64, 発行日 1999-03-31 |
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表示順 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 7 | |||||
アクセション番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | KJ00004311150 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09182276 |