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〔研究ノート〕 ムスカリン性アセチルコリン受容体の細胞膜への トラフィッキングの観察
https://swu.repo.nii.ac.jp/records/7072
https://swu.repo.nii.ac.jp/records/7072d7e7873f-3af5-4ce4-911f-b6a3457e8e8c
名前 / ファイル | ライセンス | アクション |
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②学苑12月号須賀先生 (964.3 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2021-04-16 | |||||
タイトル | ||||||
タイトル | 〔研究ノート〕 ムスカリン性アセチルコリン受容体の細胞膜への トラフィッキングの観察 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | The Export Trafficking of the Muscarinic Acetylcholine Receptors Induced by an Antagonist | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gタンパク質共役受容体(GPCR)|ムスカリン受容体|アンタゴニスト|リガンド結合|全反射照明蛍光顕微鏡|トラフィッキング|薬理学的シャペロン|フォールディング | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | G protein-coupled receptor | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | muscarinic receptor | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | antagonist | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | ligand binding | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | total internal reflection fluorescence microscope | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | trafficking | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | pharmacological chaperone | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | folding | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
須賀, 比奈子
× 須賀, 比奈子× エラート, フレデリックJ.× ソーヤー, グレゴリーW.× SUGA, Hinako× EHLERT, Frederick J.× SAWYER, Gregory W. |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | G protein-coupled receptors are cell-surface receptors, many of which have conserved motif F(x)6LL in their C-terminal intracellular region. The motif is known to function when the G protein-coupled receptors are exported from the endoplasmic reticulum to the cell surface. We reported previously that the amino acid mutations of the conserved leucines of M1 muscarinic acetylcholine receptor caused significant decrease in the cell-surface expression and significant increase in the endoplasmic reticulum expression of the mutant receptor, and that in the presence of antagonist atropine, the mutant receptor showed cell-surface expression, similar to the expression of the wild-type receptor. In this study, we investigated the export trafficking of the mutant receptor by the measurements of membrane-impermeable antagonist [3H]N-methylscopolamine binding to the cell surface, and indicated that the cell-surface expression of the mutant receptor in the presence of atropine decreased to 20% by the depletion of atropine for 3 days, and recovered to the same amount as before by the second addition of atropine. We also investigated the export trafficking of the mutant receptor using the total internal reflection fluorescence microscope, and indicated that the mutant receptor expressed time-dependently to the cell surface by the second addition of atropine. | |||||
書誌情報 |
学苑 en : Gakuen 号 962, p. 8-15, 発行日 2020-12-01 |
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出版者 | ||||||
出版者 | 昭和女子大学近代文化研究所 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1348-0103 |